{"created":"2023-06-19T11:23:01.629827+00:00","id":1274,"links":{},"metadata":{"_buckets":{"deposit":"b06fa28f-0f29-4eb0-837a-4cba7e73bc43"},"_deposit":{"created_by":12,"id":"1274","owners":[12],"pid":{"revision_id":0,"type":"depid","value":"1274"},"status":"published"},"_oai":{"id":"oai:jichi-ir.repo.nii.ac.jp:00001274","sets":["14:1747372335465:46"]},"author_link":[],"control_number":"1274","item_2_alternative_title_1":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"器質形成期のラット下顎切歯エナメル器におけるエナメル芽細胞に隣接する星状綱細胞の代用となる先行乳頭層の存在","subitem_alternative_title_language":"ja"}]},"item_2_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2023-03","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"19","bibliographicPageStart":"11","bibliographicVolumeNumber":"45","bibliographic_titles":[{"bibliographic_title":"自治医科大学紀要","bibliographic_titleLang":"ja"},{"bibliographic_title":"Jichi Medical University Journal","bibliographic_titleLang":"en"}]}]},"item_2_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Objective: Since rat incisors continue to grow for the duration of the animals’ lifespan, the rat ameloblast is an optimal candidate for an amelogenesis study. While other cells in the enamel organ are not studied well, they also help create enamel for the animal’s entire lifespan. This study aims to clarify the properties and specificity of the cells in the enamel organ of the rat incisor.\nMaterials & Methods: Mandibular incisor samples from of postnatal 2-day-old, 10-day-old, and adult rats were analyzed histologically for the following characteristics: amelogenin expression, cytokeratin 14 (K14) expression, alkaline phosphatase (ALP) activity, and periodic acid-Schiff (PAS) reaction. Gene expression of ALP (AKP2) was also detected using RT-PCR. Cells isolated from the enamel sheet of rat incisor were cultured to confirm these characteristics in vitro.\nResults: The enamel-producing organ of the rat incisor was examined histologically and indicated the presence of a cell layer above the stratum intermedium (SI) and ameloblast in the stage of enamel matrix formation. This layer had papillary layer-like histology, ALP activity, and PAS positivity. Ameloblasts beneath the layer were characterized as being in the matrix formation stage using a cell marker study. The eccentric layer was thought to be a specific entity and designated the preceding papillary layer (PPL). Specific characteristics of the PPL were maintained in vitro using cell culture.\nConclusion: The PPL is a specific component of the enamel organ in the rat incisor and possibly allows the rat to continually produce enamel.","subitem_description_type":"Abstract"}]},"item_2_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"names":[{"name":"中野, 崇文","nameLang":"ja"}]},{"names":[{"name":"杉浦-中里, 真琴","nameLang":"ja"}]},{"names":[{"name":"坂口, もも子","nameLang":"ja"}]},{"names":[{"name":"松村, 達志","nameLang":"ja"}]},{"names":[{"name":"森, 良之","nameLang":"ja"}]},{"names":[{"name":"田畑, 純","nameLang":"ja"}]}]},"item_2_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"自治医科大学","subitem_publisher_language":"ja"}]},"item_2_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1881-252X","subitem_source_identifier_type":"PISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"NAKANO, Takafumi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"NAKAZATO-SUGIURA, Makoto","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"SAKAGUCHI, Momoko","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"MATSUMURA, Tatsushi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"MORI, Yoshiyuki","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"TABATA, Makoto J","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2023-06-13"}],"displaytype":"detail","filename":"自治医科大学紀要 1881-252X(45)P11-19 2023.pdf","filesize":[{"value":"907.4 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"自治医科大学紀要 1881-252X(45)P11-19 2023","url":"https://jichi-ir.repo.nii.ac.jp/record/1274/files/自治医科大学紀要 1881-252X(45)P11-19 2023.pdf"},"version_id":"c898269e-0edf-4d1f-aa15-68e98b099bd2"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Enamel matrix formation stage","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Enamel maturation stage","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Enamel organ","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Preceding Papillary Layer","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Rat incisor","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"先行乳頭層細胞","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"ラット切歯","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"器質形成期","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"成熟期","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"エナメル器","subitem_subject_language":"ja","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Enamel Organ of Rat Incisor has Preceding Papillary Layer instead of Stellate Reticulum above the Ameloblast in the Stage of Enamel Matrix Formation","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Enamel Organ of Rat Incisor has Preceding Papillary Layer instead of Stellate Reticulum above the Ameloblast in the Stage of Enamel Matrix Formation","subitem_title_language":"en"}]},"item_type_id":"2","owner":"12","path":["46"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-06-09"},"publish_date":"2023-06-09","publish_status":"0","recid":"1274","relation_version_is_last":true,"title":["Enamel Organ of Rat Incisor has Preceding Papillary Layer instead of Stellate Reticulum above the Ameloblast in the Stage of Enamel Matrix Formation"],"weko_creator_id":"12","weko_shared_id":-1},"updated":"2025-05-28T03:24:38.012378+00:00"}